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Hsa_circ_0026344 suppresses gastric cancer progression via modulating the miR-1290/FBP2 axis

GaoChun Xiao1, TingTing Zhang2, BinBin Tan1

  • 1Department of General Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, PR China.

Histology and Histopathology
|December 14, 2022
PubMed
Abstract

Insights

Circular RNA (circRNA) hsa_circ_0026344 inhibits gastric cancer (GC) progression by downregulating miR-1290 and upregulating Fructose-1,6-bisphosphatase 2 (FBP2). This circRNA may serve as a novel therapeutic target for GC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging as critical regulators in tumorigenesis, particularly in gastric cancer (GC).
  • The specific roles of most circRNAs, including hsa_circ_0026344 (circ_0026344), in GC progression are not well understood.
  • This study investigates the functional impact of circ_0026344 on gastric cancer development.

Purpose of the Study:

  • To elucidate the role of circ_0026344 in gastric cancer (GC) progression.
  • To investigate the molecular mechanisms underlying circ_0026344's function in GC.
  • To explore circ_0026344 as a potential therapeutic target for GC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure circ_0026344, miR-1290, and Fructose-1,6-bisphosphatase 2 (FBP2) expression.
  • Cellular assays including colony formation, 5-ethynyl-2'-deoxyuridine (EdU), and transwell assays were performed to assess GC cell proliferation, migration, and invasion.
  • RNA pull-down and dual-luciferase reporter assays were employed to confirm interactions between circ_0026344, miR-1290, and FBP2. In vivo tumor growth was evaluated using a xenograft model.

Main Results:

  • Circ_0026344 expression was significantly reduced in GC cells and tissues.
  • Overexpression of circ_0026344 suppressed GC cell proliferation, migration, and invasion.
  • Circ_0026344 inhibited GC progression by upregulating FBP2 expression through the miR-1290 sponge mechanism, as confirmed by in vitro and in vivo experiments.

Conclusions:

  • Circ_0026344 functions as a tumor suppressor in gastric cancer.
  • The identified circ_0026344/miR-1290/FBP2 axis provides a novel molecular mechanism in GC pathogenesis.
  • Circ_0026344 holds promise as a potential therapeutic target for gastric cancer treatment.

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