Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neural stem cell epigenomes and fate bias are temporally coordinated during mouse cortical development.

Genes & development·2026

Same author

Z-DNA formation regulates the totipotent-like state and primes Zscan4-dependent chromatin compartmentalization.

Nature structural & molecular biology·2026

Same author

Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis.

Nature communications·2026

Same author

IceQream: Quantitative chromosome accessibility analysis using physical TF models.

Nature communications·2025

Same author

The Biodiversity Cell Atlas: mapping the tree of life at cellular resolution.

Nature·2025

Same author

Specificity and mechanism of tRNA cleavage by the AriB Toprim nuclease of the PARIS bacterial immune system.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2025

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

TATTOO-seq delineates spatial and cell type-specific regulatory programs in the developing limb.

Sébastien Bastide^1,2,3, Elad Chomsky^4,5, Baptiste Saudemont¹

¹(Epi)genomics of Animal Development, Department of Developmental and Stem Cell Biology, Institut Pasteur, Paris, France.

Science Advances

|December 14, 2022

Summary

This summary is machine-generated.

This study reveals how cells develop and organize during mouse limb formation using advanced single-cell transcriptomics. It identifies key genetic regulators of cell specialization and spatial positioning in embryogenesis.

More Related Videos

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Published on: November 17, 2016

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Related Experiment Videos

Last Updated: Jun 15, 2026

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Application of Impermeable Barriers Combined with Candidate Factor Soaked Beads to Study Inductive Signals in the Chick

Published on: November 17, 2016

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

Area of Science:

Developmental Biology
Genomics
Computational Biology

Background:

Embryogenesis relies on precise cell differentiation and spatial organization.
Understanding the genetic control of these processes is crucial for developmental biology.

Purpose of the Study:

To identify genetic programs governing cell specialization and spatial integration during mouse limb development.
To explore the interplay between cell fate and spatial positioning.

Main Methods:

Developed and applied an unbiased, spatially resolved single-cell transcriptomics method.
Analyzed gene expression patterns in developing mouse limbs.

Main Results:

Identified transcription factors regulating cell type specification and spatial position.
Revealed two interconnected regulatory systems controlling development.
Observed complex reorganization of limb development upon perturbation of spatial organizing centers, including cell loss and altered molecular identities.

Conclusions:

Multidimensional single-cell, spatially resolved atlases can decouple spatial identity from cell fate.
Uncovered interconnected genetic networks essential for organogenesis and its response to genetic alterations.