Related Experiment Video
Updated: Aug 17, 2025

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters
Vinitha Packirisamy1, Prabhu Pandurangan1
1Department of Physical Chemistry, School of Chemical Science, University of Madras, Guindy Campus, Chennai, 600025, India.
Abstract:
Atomically precise nanoclusters comprising 1-100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number of metal atoms and ligands that determines their functions and applicability. Since 1990, thiolated gold nanoclusters have been studied. The separation of monodispersed clusters was crucial and time-consuming. To address these shortcomings, several separation techniques have made it possible to separate the series of metal nanoclusters with a precise composition of metals and ligands. Among these techniques, polyacrylamide gel electrophoresis was utilized for hydrophilic cluster separation. This review shall focus on the principle, operation and application of the polyacrylamide gel electrophoresis technique to encourage a greater understanding of the characteristics and usefulness of this method.
Related Concept Videos
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

