Related Experiment Video

Updated: Aug 17, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

RNA m6A modification: Mapping methods, roles, and mechanisms in acute myeloid leukemia

Rong Yin1, Yashu Li2, Wen Tian2

  • 1Department of Hematology, Zhongnan Hospital, Wuhan University, Wuhan, People's Republic of China.

Blood Science (Baltimore, Md.)
|December 15, 2022
PubMed

Insights

N6-Methyladenosine (m6A) is crucial in regulating RNA and is key in acute myeloid leukemia (AML). This review covers m6A biology, detection methods, and potential AML treatments targeting m6A modifiers.

Area of Science:

  • Epitranscriptomics
  • Molecular Biology
  • Hematology

Background:

  • N6-Methyladenosine (m6A) is the most prevalent mRNA modification in eukaryotes.
  • m6A regulates RNA fate and plays a significant role in hematologic malignancies like acute myeloid leukemia (AML).
  • Advances in epitranscriptomics have enabled transcriptome-wide detection of m6A modifications.

Purpose of the Study:

  • To summarize recent advances in RNA m6A biology, focusing on writers, readers, and erasers.
  • To describe the development of high-throughput methods for RNA m6A mapping.
  • To discuss the role of m6A modifiers in AML and identify potential therapeutic targets.

Main Methods:

  • Review of current literature on m6A biology and its role in AML.
  • Summary of technological advancements in high-throughput m6A detection and mapping.
  • Analysis of m6A modifiers as potential therapeutic targets in AML.

Main Results:

  • m6A modification is integral to RNA regulation and implicated in AML pathogenesis.
  • High-throughput methods have significantly advanced the study of m6A.
  • m6A modifiers represent promising targets for novel AML therapies.

Conclusions:

  • Understanding m6A biology is critical for advancing AML research.
  • Targeting m6A modifiers offers a potential new avenue for AML treatment.
  • This review provides a comprehensive overview of m6A in the context of AML.
Keywords:
Acute myeloid leukemiaMappingRNA m6A modification

More Related Videos

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.8K
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.7K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.1K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.8K