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Related Experiment Video

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Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
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Granulocyte concentrate splitting does not affect phenotype and function.

Sebastian Koo1,2, Robert Offner1, Sophie-Marie Haile2

  • 1Institute for Clinical Chemistry and Laboratory Medicine, Transfusion Medicine, University Hospital Regensburg, Raubling, Germany.

Transfusion
|December 15, 2022
PubMed
Summary
This summary is machine-generated.

Splitting granulocyte concentrates (GCs) does not affect their function or viability. However, storing GCs for more than 24 hours significantly impairs their function, limiting their clinical use.

Keywords:
distributiongranulocyte concentratesgranulocytesmodified fluid gelatinneutrophilsseparationstoragetransfusion

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Area of Science:

  • Hematology
  • Transfusion Medicine
  • Cellular Immunology

Background:

  • Granulocyte concentrates (GCs) are vital for treating certain infections.
  • Current limitations in GC production and storage impact patient access.
  • Investigating methods to increase GC availability is crucial.

Purpose of the Study:

  • To evaluate the impact of splitting apheresis bags on GC viability and function.
  • To determine the effect of extended storage periods on GC quality.
  • To assess if splitting and longer storage can enhance GC availability without compromising efficacy.

Main Methods:

  • Granulocyte apheresis concentrates were split into two portions.
  • Stored GCs for 24 and 48 hours.
  • Assessed granulocyte function via cell migration, ROS production, and NETosis using live-cell imaging.
  • Measured ROS production, CD11b expression, and viability via flow cytometry.

Main Results:

  • Splitting GCs did not affect viability or function.
  • After 24 hours, most functions remained stable, but PMA-induced ROS production decreased.
  • After 48 hours, significant declines in ROS production, viability, and migration were observed.
  • Extended storage beyond 24 hours compromised key granulocyte functions.

Conclusions:

  • Splitting granulocyte concentrates is a safe method that does not impair cell function or viability.
  • Extended storage of GCs beyond 24 hours leads to a compromise in their functional capacity.
  • Transfusion of GCs should ideally occur within 24 hours of collection to ensure optimal therapeutic benefit.