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Updated: Aug 17, 2025

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
Published on: January 16, 2018
Mass-Spec, Cryo-EM and AI join forces for a close look at the transporter complex in cilia
1Department of Biology and Chemistry, Paul Scherrer Institute, Villigen PSI, Switzerland.
Abstract:
The intraflagellar transport (IFT) complex transports components between the cytoplasm and the ciliary tip. Two studies now report on the atomic structure of IFT-B, the core of IFT, using cutting-edge technology, such as cross-linking mass spectrometry (MS), cryo-electron tomography (cryo-ET) and Alphafold2-enabled AI-based folding prediction. The 3D structure of IFT-B reveals how the 15 component proteins are arranged to stabilize this gigantic complex and suggests a dynamic interplay between the proteins.
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