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Updated: Aug 17, 2025
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
Published on: March 11, 2021
A naphthalimide-tyrosine-based dicationic amphiphile for intracellular 'turn-on' simultaneous detection of ATP and
Poonam Sharma1, Sugandha Kumar1,2, Amandeep Walia3
1Department of Chemistry, UGC Centre for Advanced Studies, Guru Nanak Dev University, Amritsar, Pb, India. prabhpreet.chem@gndu.ac.in.
Abstract:
We have developed a new naphthalimide-based amphiphile (YN-1) for the simultaneous detection of ATP and CTP. In YN-1, the cationic tyrosine-linked polyamine (+2 charge, hydrophilic unit) is appended at the -peri position of naphthalimide (hydrophobic unit). YN-1 and its Boc-protected compound 4 were characterized using state-of-the-art spectroscopic and optical techniques such as NMR, IR, UV-vis and fluorescence. The fluorescence data revealed that YN-1 showed a 'turn-on' (λem = 440 nm) fluorescence response for nanomolar detection of nucleoside triphosphates such as ATP and CTP in 20% HEPES buffer-DMSO solution. YN-1 also showed a concentration-based discrimination between ATP and CTP. YN-1 has been successfully applied for bioimaging of nucleoside triphosphates in MCF-7 live cancer cells with good compatibility. Therefore, the important findings from the present work will provide insight for future development of fluorescent probes to detect various kinds of essential nucleoside triphosphates.
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