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Investigating Endobacteria that Thrive Within Mucoromycota
Alessandro Desirò1, Yusuke Takashima2, Gregory Bonito1
1Department of Plant Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 15, 2022
Summary
Mucoromycota fungi host endobacteria, impacting plant microbiomes. New cellular biology, microscopy, and molecular methods enable deeper study of these crucial fungal-bacterial interactions.
Area of Science:
- Mycology
- Microbiology
- Plant Science
Background:
- Mucoromycota are vital early-diverging fungi in plant microbiomes, including mycorrhizal fungi, endophytes, pathogens, and decomposers.
- Many Mucoromycota harbor endobacteria within their mycelia and spores, representing a significant but understudied microbial interaction.
- Investigating these endosymbiotic bacteria has been challenging, but advancements in culture-free techniques are opening new research avenues.
Purpose of the Study:
- To highlight the significance of Mucoromycota and their endobacteria in plant ecosystems.
- To present a comprehensive approach for investigating Mucoromycota and their associated endobacteria.
- To underscore the importance of studying these microbial interactions for understanding plant microbiome dynamics.
Main Methods:
- Utilizing a combination of cellular biology techniques.
- Employing advanced microscopy for visualizing fungal and bacterial structures.
- Applying molecular methods for genetic and functional analysis of endobacteria.
Main Results:
- Demonstrated the feasibility of studying Mucoromycota endobacteria using integrated techniques.
- Provided insights into the cellular and molecular characteristics of the Mucoromycota-endobacteria symbiosis.
- Established a framework for future research into this emerging field.
Conclusions:
- Mucoromycota and their endobacteria play a critical role in plant health and ecosystem functioning.
- Integrated cellular, microscopy, and molecular approaches are effective for studying these complex endosymbiotic relationships.
- Further research into Mucoromycota endobacteria will advance our understanding of plant microbiome evolution and function.
Keywords:
Bacterial–fungal interactionsBurkholderiaceae-related endobacteriaEndobacteriaMortierellaceaeMucoromycotaMycoavidus cysteinexigensMycoplasma-related endobacteriaMore Related Videos
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