Related Experiment Video

Updated: Aug 17, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

526

Long non-coding RNA TILR constitutively represses TP53 and apoptosis in lung cancer

Mika Iwai1, Taisuke Kajino1,2, Masahiro Nakatochi3

  • 1Division of Molecular Carcinogenesis, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.

Oncogene
|December 15, 2022
PubMed

Insights

A novel long non-coding RNA, TP53-inhibiting lncRNA (TILR), suppresses p53 expression in lung cancer. TILR, with PCBP2, maintains low p53 activity, preventing unnecessary apoptosis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • RNA Biology

Background:

  • Non-coding RNAs play crucial roles in lung cancer development.
  • The precise regulatory mechanisms involving non-coding RNAs and tumor suppressors like p53 are not fully understood.

Purpose of the Study:

  • To identify and characterize novel non-coding RNAs involved in lung cancer regulation.
  • To elucidate the function of a newly discovered lncRNA, TP53-inhibiting lncRNA (TILR), in controlling p53 expression.

Main Methods:

  • Proteomic analysis to identify TILR-interacting proteins.
  • RNA binding assays to determine TILR's interaction with p53 mRNA.
  • Gene silencing experiments (siRNA) to assess the functional impact of TILR and PCBP2.
  • Analysis of downstream gene expression and apoptosis induction.

Main Results:

  • Identification of a novel long non-coding RNA, TP53-inhibiting lncRNA (TILR).
  • TILR acts as a negative regulator of p53 expression, affecting p21 and MDM2.
  • TILR binds to p53 mRNA via PCBP2, suppressing p53 post-transcriptionally.
  • TILR influences p53 and Fanconi anemia pathway genes, creating a feedback loop.

Conclusions:

  • TILR constitutively inhibits p53 expression in conjunction with PCBP2.
  • This inhibition maintains low p53 transcriptional activity, preventing spurious apoptosis.
  • TILR represents a potential therapeutic target in lung cancer.

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.5K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.5K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.0K