Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The subpleural pulmonary microvasculature in newborn yak (Bos grunniens).

Veterinary research communications·2008
Same author

Experimental confirmation of potential swept source optical coherence tomography performance limitations.

Applied optics·2008
Same author

A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice.

Plant physiology·2008
Same author

[Spatial and temporal changes of palatal cell proliferation and cell apoptosis of retinoic acid induced mouse cleft palate in different embryonic stages].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2008
Same author

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Developmental and comparative immunology·2008
Same author

Non-Gaussian statistics and superdiffusion in a driven-dissipative dusty plasma.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Aug 17, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Research on partially coherent spatial light interference microscopy.

Zhifang Li, Bin Liu, Dinghui Tan

    Optics Express
    |December 16, 2022
    PubMed
    Summary
    This summary is machine-generated.

    This study enhances spatial light interference microscopy (SLIM) by introducing a double phase shift method. This improves reconstruction accuracy for microscopic imaging, reducing errors in samples like red blood cells.

    More Related Videos

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.4K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.0K

    Related Experiment Videos

    Last Updated: Aug 17, 2025

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.1K
    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.4K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.0K

    Area of Science:

    • Optical microscopy
    • Interference microscopy
    • Biophysics

    Background:

    • Spatial Light Interference Microscopy (SLIM) is a technique for quantitative phase imaging.
    • Existing SLIM methods often assume a default phase of 0 for direct light, leading to inaccuracies.
    • Improving the phase calculation is crucial for accurate SLIM reconstructions.

    Purpose of the Study:

    • To rigorously deduce the principle of SLIM based on partial coherence theory.
    • To improve the calculation method of SLIM by addressing the default phase assumption.
    • To experimentally demonstrate a novel phase shift method for enhanced SLIM accuracy.

    Main Methods:

    • Deduction of SLIM principles using partial coherence theory.
    • Development and implementation of a double four-step phase shift method.
    • Experimental validation using oil-immersed microspheres and red blood cells.

    Main Results:

    • The proposed double four-step phase shift method reduces the relative error in oil-immersed microsphere reconstruction to approximately 3.7%.
    • For red blood cell reconstruction, the relative error is reduced to about 13%.
    • The improved method demonstrates significantly higher accuracy compared to standard SLIM.

    Conclusions:

    • The double four-step phase shift method effectively overcomes the limitation of the default phase assumption in SLIM.
    • This enhanced SLIM technique offers improved quantitative phase imaging capabilities.
    • The findings have implications for high-accuracy microscopic analysis in various scientific fields.