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Curcumin suppresses lung cancer progression via circRUNX1 mediated miR-760/RAB3D axis

Xiaodan Wu1, Hong Chen2, Na Liu3

  • 1Department of Traditional Chinese Medicine, Central Hospital Affiliated to Shenyang Medical College, Shenyang, China.

Thoracic Cancer
|December 16, 2022
PubMed
Abstract

Insights

Curcumin, a natural compound, inhibits lung cancer (LC) progression. It acts through the circRUNX1/miR-760/RAB3D pathway, reducing cell proliferation and metastasis while promoting apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Natural Products Chemistry

Background:

  • Lung cancer (LC) remains a leading cause of cancer-related mortality worldwide.
  • Curcumin, a polyphenol derived from turmeric, exhibits promising anticancer properties.
  • Understanding the molecular mechanisms underlying curcumin's effects in LC is crucial for therapeutic development.

Purpose of the Study:

  • To elucidate the molecular mechanism by which curcumin regulates lung cancer (LC) progression.
  • To investigate the role of the circRUNX1/miR-760/RAB3D axis in curcumin's anti-LC effects.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure circRUNX1, miR-760, and RAB3D expression.
  • Cellular assays including CCK8, colony formation, flow cytometry, wound healing, and Transwell assays to assess proliferation, apoptosis, migration, and invasion.
  • Western blot analysis for protein expression.
  • Dual-luciferase reporter assays to confirm RNA interactions.
  • In vivo studies using LC xenograft models in nude mice.

Main Results:

  • CircRUNX1 was found to be upregulated in LC tissues and its expression was inhibited by curcumin.
  • Curcumin treatment significantly reduced LC cell proliferation, metastasis, and induced apoptosis; these effects were reversed by circRUNX1 overexpression.
  • MiR-760 was identified as a direct target of circRUNX1, mediating the effects of circRUNX1 on curcumin-treated LC cells.
  • RAB3D was confirmed as a target of miR-760, and its knockdown reversed the pro-progression effects of miR-760 inhibition in curcumin-treated LC cells.

Conclusions:

  • Curcumin suppresses lung cancer progression through the circRUNX1/miR-760/RAB3D molecular axis.
  • This study identifies a novel regulatory pathway for curcumin's anticancer activity in lung cancer.
  • Targeting the circRUNX1/miR-760/RAB3D axis may represent a potential therapeutic strategy for lung cancer treatment.
Keywords:
RAB3DcircRUNX1curcuminlung cancermiR-760

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