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Related Experiment Video

Updated: Aug 17, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

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Published on: April 18, 2025

Hep3Gel: A Shape-Shifting Extracellular Matrix-Based, Three-Dimensional Liver Model Adaptable to Different Culture

Giuseppe Guagliano¹, Cristina Volpini², Lorenzo Sardelli¹

¹Department of Chemistry, Materials, and Chemical Engineering "G. Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133Milan, Italy.

ACS Biomaterials Science & Engineering

|December 16, 2022

Summary

This summary is machine-generated.

This study introduces Hep3Gel, a novel hydrogel for 3D liver models that improves cell survival and mimics the liver

Keywords:

3D bioprinting 3D cell cultures alginate bioink decellularized hepatic tissue internal crosslinking

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Published on: August 27, 2016

Area of Science:

Biomaterials Science
Hepatology
Tissue Engineering

Background:

Drug-induced hepatotoxicity frequently halts clinical trials.
Accurate in vitro liver models are essential for drug development and understanding liver function.
Current 2D and custom chamber models have limitations in replicating the liver's complex environment.

Purpose of the Study:

To develop Hep3Gel, a hybrid alginate-extracellular matrix (ECM) hydrogel for 3D in vitro liver models.
To create a material that reproduces the liver's chemomechanical niche.
To engineer a versatile hydrogel adaptable to various manufacturing techniques.

Main Methods:

Extraction and powdering of porcine liver ECM.
Embedding ECM into alginate hydrogels to create Hep3Gel.
Tuning viscoelastic properties to match hepatic biomechanics.
Characterizing Hep3Gel's shape-shifting abilities (self-spreading and 3D-bioprinting).
Assessing cell viability in Hep3Gel compared to control hydrogels.

Main Results:

Hep3Gel successfully mimics the liver's chemomechanical niche.
The hydrogel exhibits shape-shifting properties, enabling self-spreading and 3D-bioprinting.
Addition of ECM significantly enhanced cell survival, doubling it after 4 days.
Rheological evaluations confirmed Hep3Gel's printability as a bioink.

Conclusions:

Hep3Gel is a promising biomaterial for advanced 3D in vitro liver models.
This hydrogel can support drug development by providing a more physiologically relevant platform.
Further development of Hep3Gel could significantly advance research in hepatotoxicity and liver disease modeling.