Point-of-care assay platform for uric acid, urea, and triglycerides with a microfluidic paper device (μPAD)
M D Fernández-Ramos1, M Bolaños-Bañuelos2, L F Capitán-Vallvey1
1ECsens. Department of Analytical Chemistry, University of Granada, Granada, 18071, Spain; Unit of Excellence in Chemistry Applied to Biomedicine and the Environment of the University of Granada, Spain.
Abstract:
A microfluidic biosensor paper-based analytical device, or μPAD, was developed as a point-of-care assay platform for the simultaneous determination of three clinically important markers: uric acid, urea, and triglycerides. To achieve a simultaneous determination, thermosensitive valves based on N-isopropylacrylamide polymers controlled the flow of fluid in the μPAD. The evaluation of the analytical characteristics of the μPAD was done using a photographic camera at room temperature. The detection limits were 4.5·10-5, 2.5·10-3, and 1.5·10-3 mg mL-1, for uric acid, urea, and triglycerides, respectively, and the precision expressed as relative standard deviation (% RSD) 3.2, 1.2, and 1.6%. The obtained μPAD devices were validated with real human plasma samples, demonstrating high accuracy and precision. Multiple analyte point-of-care tests such as those developed here have excellent characteristics, being easy to manufacture, cost-effective, easy to use, and highly sensitive.
