Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental triggers of a Microcystis (Cyanophyceae) bloom in an artificial lagoon of Hangzhou Bay, China.

Marine pollution bulletin·2018
Same author

Quantitative evaluation of retinal artery occlusion using optical coherence tomography angiography: A case report.

Medicine·2018
Same author

Multibandgap quantum dot ensembles for solar-matched infrared energy harvesting.

Nature communications·2018
Same author

Butylamine-Catalyzed Synthesis of Nanocrystal Inks Enables Efficient Infrared CQD Solar Cells.

Advanced materials (Deerfield Beach, Fla.)·2018
Same author

A secretory hexokinase plays an active role in the proliferation of <i>Nosema bombycis</i>.

PeerJ·2018
Same author

Discovery of furyl/thienyl β-carboline derivatives as potent and selective PDE5 inhibitors with excellent vasorelaxant effect.

European journal of medicinal chemistry·2018

Related Experiment Video

Updated: Aug 17, 2025

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

3.3K

What has single-cell transcriptomics taught us about long non-coding RNAs in the ventricular-subventricular zone?

Jemima Becker1, Bin Sun1, Farah Alammari2

  • 1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.

Stem Cell Reports
|December 16, 2022
PubMed
Summary

This study reveals long non-coding RNAs (lncRNAs) in the brain's neural stem cell niche. These lncRNAs are crucial for stem cell activation and neurogenesis, impacting brain health and disease.

Keywords:
V-SVZadult neurogenesisepigeneticslncRNAneural stem cellsscRNA-seq

More Related Videos

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow
14:33

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

Published on: May 6, 2010

42.1K
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

9.3K

Related Experiment Videos

Last Updated: Aug 17, 2025

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

3.3K
The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow
14:33

The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow

Published on: May 6, 2010

42.1K
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

9.3K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) regulate gene networks but their functions in the brain's neural stem cell niche are poorly understood.
  • The ventricular-subventricular zone (V-SVZ) is a key postnatal neural stem cell niche where lncRNAs are transcribed.
  • Aging impairs V-SVZ neurogenesis due to stem cell depletion and reduced activation.

Purpose of the Study:

  • To analyze V-SVZ transcriptomics and identify lncRNAs involved in neural stem cell activation and differentiation.
  • To investigate the role of lncRNAs in V-SVZ neurogenesis during aging and in disease contexts.
  • To predict novel V-SVZ lncRNAs regulating stem cell function.

Main Methods:

  • Pooling and analysis of existing single-cell RNA-sequencing (scRNA-seq) data from the V-SVZ.
  • Integration of transcriptomic data with epigenetic and genetic information.
  • Prediction of lncRNAs regulating stem cell activation and lineage progression.

Main Results:

  • Consistent lncRNA expression patterns were observed during V-SVZ stem cell activation, lineage progression, and aging.
  • Several V-SVZ lncRNAs were predicted to regulate stem cell activation and differentiation.
  • Some identified lncRNAs align with known epigenetic mechanisms, while many represent novel targets.

Conclusions:

  • This study identifies key lncRNAs in the V-SVZ that are critical for neural stem cell function.
  • These lncRNAs play significant roles in neurogenesis, aging, and brain diseases.
  • Further investigation of these novel lncRNAs is warranted to understand their precise mechanisms in health and disease.