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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
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Modulation of peroxisomal compartment by Epstein-Barr virus
Omkar Indari1, Annu Rani1, Budhadev Baral1
1Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Simrol, Indore, 453552, India.
Microbial Pathogenesis
|December 16, 2022
Summary
Epstein-Barr virus (EBV) alters peroxisomal protein expression and lipid metabolism. EBV infection upregulates PEX19 and changes lipid content, impacting cellular function and disease pathology.
Area of Science:
- Virology
- Cell Biology
- Biochemistry
Background:
- Viruses manipulate cellular functions for infection.
- Peroxisomal compartment exploitation by viruses is increasingly recognized.
- Epstein-Barr virus (EBV) is associated with cancers and neurological disorders, but its effect on peroxisomes is unknown.
Purpose of the Study:
- To investigate the impact of EBV infection on peroxisomal protein modulation.
- To analyze changes in peroxisomal proteins and lipid content in EBV-transformed and acutely infected cells.
Main Methods:
- Gene expression analysis of peroxisomal proteins via qPCR.
- Lipid content quantification using fluorescence microscopy and biochemical assays.
- Utilized EBV-positive and negative Burkitt lymphoma cell lines and EBV-infected peripheral blood mononuclear cells.
Main Results:
- Distinct regulation of peroxisomal proteins observed in EBV-transformed and acutely infected cells.
- Significant upregulation of PEX19 in EBV-infected cells.
- EBV-transformed cells showed decreased lipid abundance, while acute infection led to increased lipid levels.
Conclusions:
- EBV infection significantly alters peroxisomal protein expression and lipid metabolism.
- Differential regulation of peroxisomal proteins highlights peroxisome manipulation by EBV.
- Further research into EBV-peroxisome interactions may reveal novel pathways in EBV-mediated diseases.
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