Silver nanoparticles modified hFGF2-linking camelina oil bodies accelerate infected wound healing

Bacterial infection wounds are common in life. At present, although various wound materials have shown antibacterial activity, there is a lack of overall strategy to promote wound healing. Therefore, it is necessary to develop multifunctional wound materials. In this study, silver nanoparticles (Ag NPs) modified camelina oil bodies (OB) which surface covalently bonded human fibroblast growth factor 2 (Ag NPs-hFGF2-OB) were designed for the treatment of bacterial infection wounds. The prepared Ag NPs-hFGF2-OB not only act as an antibacterial agent to realize sterilization, but also act as a tissue repair agent that effectively promotes wound healing. Ag⁺ was reduced in situ to Ag NPs by ascorbic acid, and the activity of hFGF2 protein was not affected after hFGF2-OB was modified by Ag NPs, which displaying broad apectrum antibacterial ability for both S. aureus and E. coli, with an antibacterial rate of more than 70 % (the concentration of Ag NPs was 20 μg/mL, the hFGF2 protein concentration was 20 µg/mL). Ag NPs-hFGF2-OB can effectively promote the migration of NIH/3T3 cells, showing good biocompatibility. The mouse bacterial infection wound model experiments proved that the wound healing rate of Ag NPs-hFGF2-OB group (the concentration of Ag NPs was 20 μg/mL, the hFGF2 protein concentration was 20 µg/mL) was much higher than other treatment groups, especially on the 7th day after treatment, the wound healing rate reached 71.71 ± 2.38 %, while the healing rate of other treatment groups were only 34.54 ± 1.10 %, 37.08 ± 2.85 % and 47.99 ± 2.01 %. Therefore, Ag NPs-hFGF2-OB, which can inhibit bacterial growth, promotes collagen deposition, granulation tissue regeneration and angiogenesis without any significant toxicity, shows good potential for application in the repair of bacterial infection wounds.