Chloroplast-boosted photodynamic therapy for effective drug-resistant bacteria killing and biofilm ablation

Due to the misuse of various antibiotics, the problem of bacterial resistance has become more serious worldwide, and the associated diseases have significantly increased the medical burden of society. Antimicrobial photodynamic therapy (PDT) has received widespread attention because of its safety, efficiency, and facile implementation. Here, we report an oxygen-supply antibacterial agent (Ce6@CS/CP), which could enhance the efficacy of antibacterial PDT via photosynthesis of O₂. Ce6@CS/CP displayed a robust interaction with bacteria, hence facilitating the delivery efficiency of Ce6. In vitro experiments demonstrated that the photodynamic bactericidal potency of Ce6@CS/CP was remarkably greater than that of free Ce6. Furthermore, Ce6@CS/CP also exhibited superior significant antibiofilm activity to free Ce6. As a live oxygen-supply antibacterial agent, Ce6@CS/CP possesses excellent bacteria delivery ability of Ce6 and could enhance the potency of antibacterial PDT by photosynthesis, offering a new strategy for fighting against drug-resistant bacteria.