Soil ammonium (NH₄⁺) toxicity thresholds for restoration grass species

Ionic rare earth mining has resulted in large amounts of bare soils, and revegetation success plays an important role in mine site rehabilitation and environmental management. However, the mining soils still maintain high NH₄⁺ concentrations that inhibit plant growth and NH₄⁺ toxicity thresholds for restoration plants have not been established. Here we investigated the NH₄⁺ toxicological effects and provided toxicity thresholds for grasses (Lolium perenne L. and Medicago sativa L.) commonly used in restoration. The results show that high NH₄⁺ concentration not only reduces the plant biomass and soluble sugars in leaves but also increases the H₂O₂ and MDA content, and SOD, POD, and GPX activities in roots. The SOD activities and root biomass can be adopted as the most NH₄⁺ sensitive biomarkers. Six ecotoxicological endpoints (root biomass, soluble sugars, proline, H₂O₂, MDA, and GSH) of ryegrass, eight ecotoxicological endpoints (root biomass, soluble sugars, proline, MDA, SOD, POD, GPX, and GSH) of alfalfa were selected to determine the threshold concentrations. The toxicity thresholds of NH₄⁺ concentrations were proposed as 171.9 (EC₅), 207.8 (EC₁₀), 286.6 (EC₂₅), 382.3 (EC₅₀) mg kg^-1 for ryegrass and 171.9 (EC₅), 193.2 (EC₁₀), 234.7 (EC₂₅), 289.6 (EC₅₀) mg kg^-1 for alfalfa. The toxicity thresholds and the relation between plant physiological indicators and NH₄⁺ concentrations can be used to assess the suitability of the investigated plants for ecological restoration strategies.