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PIK3R2 predicts poor outcomes for patients with melanoma and contributes to the malignant progression via

Jianguo Wang1, Shizhong Cai2,3, Qianwei Xiong4,3

  • 1Department of Surgery, Nanjing Pukou Central Hospital (Pukou Branch Hospital of Jiangsu Province Hospital), Nanjing, 211800, Jiangsu, People's Republic of China.

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
|December 17, 2022
PubMed
Abstract

Insights

Phosphoinositide-3-kinase regulatory subunit 2 (PIK3R2) drives melanoma progression by activating the PI3K/AKT/NF-κB pathway. Targeting PIK3R2 may offer a new therapeutic strategy for melanoma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Melanoma is a significant global health concern.
  • The role of Phosphoinositide-3-kinase regulatory subunit 2 (PIK3R2) in melanoma pathogenesis is not fully understood.

Purpose of the Study:

  • To elucidate the function and mechanism of PIK3R2 in melanoma.
  • To investigate PIK3R2 as a potential therapeutic target for melanoma.

Main Methods:

  • Quantitative real-time PCR and Western blot to assess PIK3R2 expression in melanoma tissues and cells.
  • Immunohistochemistry to correlate PIK3R2 expression with melanoma stage.
  • In vitro and in vivo assays (loss/gain-of-function, CCK-8, flow cytometry, Transwell, xenograft model) to evaluate PIK3R2's functional impact.
  • Rescue experiments to determine the underlying molecular mechanism.

Main Results:

  • PIK3R2 expression is elevated in melanoma tissues and cells, correlating with advanced stage (Stage IV).
  • PIK3R2 knockdown inhibits melanoma cell proliferation, invasion, and epithelial-mesenchymal transition, while promoting apoptosis.
  • PIK3R2 overexpression exhibits opposite effects.
  • PIK3R2 activates the PI3K/AKT/NF-κB pathway, promoting melanoma progression and tumor growth in vivo.

Conclusions:

  • PIK3R2 exacerbates melanoma progression through the PI3K/AKT/NF-κB pathway.
  • PIK3R2 represents a promising therapeutic target for melanoma treatment.
Keywords:
ApoptosisInvasionMelanomaPI3K/AKT/NF-κBPIK3R2Proliferation

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