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Mouse glutathione S-transferase Ya subunit: gene structure and sequence.
V Daniel1, R Sharon, Y Tichauer
1Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
DNA (Mary Ann Liebert, Inc.)
|August 1, 1987
Summary
Researchers isolated and sequenced a mouse glutathione S-transferase gene (Ya subunit). This gene shares high homology with rat liver Ya mRNA, revealing conserved intronic and pre-mRNA sequences between species.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Glutathione S-transferases (GSTs) are crucial enzymes involved in detoxification.
- The Ya subunit is a major isoenzyme in various species.
Purpose of the Study:
- To isolate and characterize the mouse glutathione S-transferase Ya subunit gene.
- To compare the mouse Ya gene with its rat counterpart at the sequence level.
Main Methods:
- Gene isolation and sequencing (genomic and cDNA).
- Sequence analysis and comparison between mouse and rat genes.
- Identification of promoter elements and mRNA characteristics.
Main Results:
- The mouse Ya gene spans approximately 11 kb with seven exons, encoding an 841-nucleotide mRNA.
- High homology was observed between mouse and rat Ya mRNA coding sequences.
- Sequence conservation was noted in introns and pre-mRNA regions between mouse and rat Ya genes.
Conclusions:
- The mouse Ya gene structure and sequence are highly similar to the rat Ya gene.
- Conserved sequences in introns and pre-mRNA suggest functional importance in gene regulation or processing.