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Updated: Aug 17, 2025

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Dysregulation of miR-193a serves as a potential contributor to MS pathogenesis via affecting RhoA and Rock1

Nasim Saeidi1, Hadi Goudarzvand2, Hamed Mohammadi3

  • 1DNA Laboratory, Analytical Laboratories, Hamilton, New Zealand.

Multiple Sclerosis and Related Disorders
|December 18, 2022
PubMed
Abstract

Insights

Reduced miR-193a levels in multiple sclerosis (MS) patients correlate with increased RhoA and ROCK1 expression, indicating potential as a diagnostic and therapeutic biomarker for MS.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Multiple sclerosis (MS) is a chronic central nervous system inflammatory disease.
  • MicroRNAs (miRNAs) are key regulators and potential biomarkers for diseases.
  • Identifying novel biomarkers for MS early detection and treatment is crucial.

Purpose of the Study:

  • To investigate the role of miR-193a in multiple sclerosis (MS).
  • To explore miR-193a as a potential diagnostic and prognostic biomarker for MS.
  • To assess miR-193a's therapeutic potential in MS.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blotting were used to assess miR-193a, RhoA, and ROCK1 expression in MS patients.
  • miR-193a mimic transfection was performed in MS-derived peripheral blood mononuclear cells (PBMCs).
  • Correlation analysis between miR-193a levels and clinicopathological features was conducted.

Main Results:

  • miR-193a expression was significantly decreased in MS patients' PBMCs compared to controls.
  • RhoA and ROCK1 expression levels were elevated in MS patients and reduced upon miR-193a transfection.
  • Down-regulation of miR-193a correlated with increased C-reactive protein (CRP), cerebrospinal fluid (CSF) IgG positivity, and Multiple Sclerosis Severity Score (MSSS).

Conclusions:

  • miR-193a down-regulation modulates RhoA and ROCK1 expression in MS, contributing to disease progression.
  • miR-193a and its targets (RhoA, ROCK1) show promise as diagnostic, prognostic, and therapeutic targets for MS.
  • This study highlights miR-193a as a potential non-invasive biomarker for MS.
Keywords:
Multiple sclerosisROCK1RhoAmiR-193a

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