Characteristics of soil N₂O emission and N₂O-producing microbial communities in paddy fields under elevated CO₂...

The effects of elevated carbon dioxide (CO₂) concentration (e[CO₂]) on nitrous oxide (N₂O) emissions from paddy fields and the microbial processes involved in N₂O emissions have recently received much attention. Ammonia-oxidizing microorganisms and denitrifying bacteria dominate the production of N₂O in paddy soils. To better understand the dynamics of N₂O production under e[CO₂], a field experiment was conducted after five years of CO₂ fumigation based on three treatments: CK (ambient atmospheric CO₂), T1 (CK + increase of 40 ppm per year until 200 ppm), and T2 (CK + 200 ppm). N₂O fluxes, soil physicochemical properties, and N₂O production potential were quantified during the rice-growth period. The functional gene abundance and community composition of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were analyzed using quantitative polymerase chain reaction (qPCR) and those of ammonia-denitrifying bacteria (nirS- and nirK-type) were analyzed using Illumina MiSeq sequencing. N₂O emissions decreased by 173% and 41% under the two e[CO₂] treatments during grain filling and milk ripening, respectively (P < 0.05). N₂O emissions increased by 279% and 172% in the T2 treatment compared with T1 during the tillering and milk-ripening stages, respectively (P < 0.05). Furthermore, the N₂O production potential was significantly higher in the CK treatment than in T1 and T2 during the elongation stage. The N₂O production potential and abundance of AOA amoA genes in T1 treatment were significantly lower than those in CK treatment during the high N₂O emission phase caused by mid-season drainage (P < 0.05). Although nirK- and nirS-type denitrifying bacteria community structure and diversity did not respond significantly (P > 0.05) to e[CO₂], the abundance of nirK-type denitrifying bacteria significantly affected the N₂O flux (P < 0.05). Linear regression analysis showed that the N₂O production potential, AOA amoA gene abundance, and nirK gene abundance explained 47.2% of the variation in N₂O emissions. In addition, soil nitrogen (N) significantly affected the nirK- and nirS-type denitrifier communities. Overall, our results revealed that e[CO₂] suppressed N₂O emissions, which was closely associated with the abundance of AOA amoA and nirK genes (P < 0.05).