Related Experiment Video
Updated: Aug 16, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Characterization of the complete chloroplast genome of Begonia handelii
Xiaozhen Zhu1, Xinghua Hu1, Shixun Huang1
1Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, China.
Abstract:
Begonia is the fifth largest genus of angiosperms in the world, and Begonia handelii is a member of the Begonia(Begoniaceae), and is one of the few species with floral fragrance in this genus. However, the chloroplast genome structure and phylogenetic relationship of this species is still unclear. In this study, the chloroplast genome of B. handelii was sequenced by Illumina HiSeq X platform, and the phylogenetic relationship of this species in Begonia was analyzed with related species. The whole chloroplast genome of B. handelii is 169,406 bp in size, which consist one large single-copy region (LSC) with 95,403 bp, one small single-copy region (SSC) with 20,089 bp, and two inverted repeat regions (IR) with 26,957 bp. The GC content of this chloroplast genome is 35.6%. Moreover, 140 genes were found in the chloroplast of B. handelii, including 90 protein-coding genes, 8 rRNA genes, 42 tRNA genes. Phylogenetic analysis showed that B. handelii is closed to B. coptidifolia and B. pulchrifolia. This study lays the foundation for further research on the chloroplast genome evolution of B. handelii chloroplasts.
More Related Videos
12:33Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
12:01A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Related Concept Videos
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
The Anatomy of Chloroplasts
Structure of...
Export of Mitochondrial and Chloroplast Genes
Anatomy of Chloroplasts
Non-nuclear Inheritance
Protein Transport to the Inner Chloroplast Membrane