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Updated: Aug 16, 2025
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Published on: December 21, 2019
Extracellular Matrix Microparticles Improve GelMA Bioink Resolution for 3D Bioprinting at Ambient Temperature
Zachary Galliger1, Caleb D Vogt2, Haylie R Helms3
1Biomedical Engineering Graduate Program, University of Minnesota, Minneapolis, MN.
Introduction:
Current bioinks for 3D bioprinting, such as gelatin-methacryloyl, are generally low viscosity fluids at room temperature, requiring specialized systems to create complex geometries.
Methods And Results:
Adding decellularized extracellular matrix microparticles derived from porcine tracheal cartilage to gelatin-methacryloyl creates a yield stress fluid capable of forming self-supporting structures. This bioink blend performs similarly at 25°C to gelatin-methacryloyl alone at 15°C in linear resolution, print fidelity, and tensile mechanics.
Conclusion:
This method lowers barriers to manufacturing complex tissue geometries and removes the need for cooling systems.
