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Updated: Aug 16, 2025
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
Published on: March 20, 2020
Inhibitory effects of postbiotic consisting sonication-killed Bifidobacterium bifidum on experimental triple negative
Farzaneh Nazari1, Parvaneh Jafari1, Bizhan Nomanpour2
1Department of Microbiology, Faculty of Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
Background And Objectives:
Breast cancer is the second leading cause of death and one of the most common malignancies among women in the world. The aim of this study was to investigate the preventive effects of postbiotic consisting of sonicated Bifidobacterium bifidum cells on triple negative breast cancer.
Materials And Methods:
Thirty-six female BALB/c mice aged 5-7 weeks were randomly divided into 3 groups (n=12): Ctrl-, healthy mice; Ctrl+, mice with breast cancer with no treatment; and Postbiotic, mice with orally gavage postbiotic before and after 4T1 cell line transplantation. Cancer progress and the effects of postbiotic were assayed by histological, immunohistochemical and gene expression quantification.
Results:
The histological results showed that administration postbiotic consisting of B. bifidum significantly decreased carcinogenesis in terms of tumor incidence, multiplicity and volume. The tumor progress was suppressed by oral intake of B. bifidum as showed by p53 and Ki-67 expression. Furthermore, Oral intake of postbiotic resulted in extended survival of mice and inhibited sever weigh loss.
Conclusion:
Pretreatment with sonication killed B. bifidum, as a postbiotic, inhibited breast cancer progress and malignancy.
