Related Experiment Video

Updated: Aug 16, 2025

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
08:51

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Published on: June 25, 2015

9.3K

Cellular functions of the ClpP protease impacting bacterial virulence

Mazen E Aljghami1, Marim M Barghash1, Emily Majaesic2

  • 1Department of Biochemistry, University of Toronto, Toronto, ON, Canada.

Frontiers in Molecular Biosciences
|December 19, 2022
PubMed

Insights

The ClpXP chaperone-protease complex is vital for bacterial survival and virulence. Its disruption impairs pathogenic bacteria

Area of Science:

  • Molecular biology
  • Microbiology
  • Biochemistry

Background:

  • Proteostasis is essential for cellular function, maintaining protein homeostasis.
  • The ClpXP complex, comprising ClpX unfoldase and ClpP protease, degrades targeted proteins in prokaryotes and eukaryotes.
  • ClpXP substrates are involved in diverse cellular processes, including DNA repair, metabolism, and gene regulation.

Purpose of the Study:

  • To provide an overview of ClpXP functions in bacterial virulence.
  • To highlight the role of ClpXP substrates in modulating pathogenicity.
  • To discuss examples from human pathogens.

Main Methods:

  • Literature review and synthesis of existing research on ClpXP.
  • Analysis of studies investigating ClpXP's impact on bacterial virulence factors.
  • Compilation of data from cell and animal infection models.

Main Results:

  • ClpXP disruption significantly affects bacterial stress responses and biofilm formation.
  • Loss of ClpXP function reduces the production of virulence effector proteins.
  • Impaired ClpXP activity leads to decreased pathogenicity in various infection models.

Conclusions:

  • The ClpXP complex is a critical determinant of bacterial virulence.
  • Targeting ClpXP presents a potential strategy for combating bacterial infections.
  • Understanding ClpXP's role is crucial for developing novel antimicrobial therapies.
Keywords:
ATP-dependent proteasesClpP proteasepathogenesissubstratesvirulence

More Related Videos

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes14:05

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Published on: September 19, 2013

9.9K
Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

50

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
67
Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
12.6K
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
116
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.2K
Antimicrobial Proteins01:23

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
2.8K
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
970