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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
Published on: November 30, 2022
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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
John R Klem1, Raèden Gray1, C Ben Lovely2
1Alcohol Research Center, Department of Biochemistry and Molecular Genetics, University of Louisville.
Journal of Visualized Experiments : Jove
|December 19, 2022
Summary
Zebrafish models offer insights into fetal alcohol spectrum disorders (FASD) by enabling precise ethanol exposure studies. A new Tol2 transgenesis system simplifies creating transgenic zebrafish for this research.
Area of Science:
- Developmental biology
- Toxicology
- Genetics
Background:
- Fetal alcohol spectrum disorders (FASD) present complex developmental defects and cognitive impairments from prenatal ethanol exposure.
- Animal models are crucial for understanding ethanol-induced developmental abnormalities.
- Zebrafish are a valuable model due to conserved genetics, development, and experimental advantages like transparency and external fertilization.
Purpose of the Study:
- To describe a flexible Tol2 transgenesis system and protocol for generating transgenic zebrafish.
- To facilitate research on ethanol-induced developmental defects using zebrafish models.
Main Methods:
- Utilizing the Tol2 transposon system with a multisite Gateway cloning approach.
- Assembling Tol2 transposon-based transgenic constructs for zebrafish.
Main Results:
- The Tol2 system provides a modular and efficient method for creating transgenic zebrafish.
- The described protocol simplifies the generation of transgenic constructs for ethanol exposure studies.
Conclusions:
- The Tol2 transgenesis system enhances the utility of zebrafish as a model for studying FASD.
- This approach facilitates genetic manipulation and precise ethanol exposure in zebrafish development research.
