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Related Experiment Video

Updated: Aug 16, 2025

Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats
04:16

Intraoperative Visualization of Subretinal Injection and Retinal Detachment in Rats

Published on: March 7, 2025

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Development of a Surgical Technique for Subretinal Implants in Rats.

Juan Carlos Martinez Camarillo1, Yuntao Hu2, Biju B Thomas3

  • 1USC Roski Eye Institute, Keck School of Medicine of the University of Southern California; USC Dr. Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, University of Southern California; juan.martinez@med.usc.edu.

Journal of Visualized Experiments : Jove
|December 19, 2022
PubMed
Summary

This study presents a cost-efficient surgical protocol for transplanting implants into the subretinal space in rats, crucial for preclinical research in retinal degeneration therapies like age-related macular degeneration (AMD). The method ensures reproducible delivery for advancing regenerative medicine.

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Area of Science:

  • Ophthalmology and Regenerative Medicine
  • Preclinical Research Models

Background:

  • Retinal degeneration, including age-related macular degeneration (AMD), is a major cause of global blindness.
  • Stem cell-based therapies are a promising avenue for regenerative medicine in treating AMD.
  • Rodent models are vital for retinal degeneration research, but surgical delivery of subretinal implants is challenging due to anatomical differences.

Purpose of the Study:

  • To provide a detailed, user-friendly surgical protocol for transplanting implants into the subretinal space in rats.
  • To establish a cost-efficient and reproducible method for preclinical studies.
  • To overcome anatomical challenges in rodent models for mimicking human therapeutic delivery.

Main Methods:

  • A miniaturized, human-sized implant is prepared.
  • An external approach bypasses intravitreal delivery, using a scleral incision and paracentesis.
  • Balanced salt solution (BSS) injection induces focal retinal detachment (RD) for implant insertion.
  • Spectral domain optical coherence tomography (SD-OCT) is used for post-operative assessment of subretinal implant placement and stability.

Main Results:

  • The protocol details critical steps for successful subretinal implantation in rats.
  • The described surgical technique is designed for cost-efficiency and reproducibility.
  • Post-operative SD-OCT confirms accurate implant placement and stability in the subretinal space.

Conclusions:

  • This protocol offers a standardized and accessible method for subretinal implantation in rat models.
  • It facilitates preclinical evaluation of regenerative therapies for retinal degeneration.
  • The technique aids in advancing translational research for conditions like AMD.