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Plasma exosomal miR-199a-3p downregulates cell proliferation and migration in Hirschsprung's disease by targeting

Yu Daiyue1, Yang Yang1, Huang Zhaorong1

  • 1Department of Pediatric Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China.

Pediatric Surgery International
|December 19, 2022
PubMed
Abstract

Insights

Plasma exosomal miR-199a-3p is upregulated in Hirschsprung's disease (HSCR) and inhibits cell growth by targeting mTOR. This microRNA is a potential diagnostic marker and therapeutic target for HSCR.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Plasma exosomal microRNAs are potential disease biomarkers.
  • Exosomal microRNAs in Hirschsprung's disease (HSCR) remain largely uncharacterized.
  • This study investigates miR-199a-3p in HSCR pathogenesis.

Purpose of the Study:

  • To analyze miRNA profiles in HSCR.
  • To elucidate the mechanism of miR-199a-3p in HSCR development.
  • To identify potential diagnostic biomarkers for HSCR.

Main Methods:

  • Plasma exosome isolation and miRNA sequencing.
  • Cell proliferation and migration assays (CCK-8, Transwell).
  • Bioinformatic analysis (GO, KEGG, PPI) and target validation (PCR, Western blot) for miR-199a-3p and mTOR.

Main Results:

  • miR-199a-3p was upregulated in plasma exosomes and HSCR tissues.
  • miR-199a-3p inhibited cell proliferation and migration in vitro.
  • mTOR was identified as a target of miR-199a-3p, showing downregulation and a negative correlation in HSCR tissues. mTOR partially reversed miR-199a-3p's effects.

Conclusions:

  • miR-199a-3p suppresses cell growth and motility by targeting mTOR.
  • Plasma exosomal miR-199a-3p is a crucial diagnostic marker for HSCR.
  • miR-199a-3p represents a potential therapeutic target for HSCR.
Keywords:
ExosomesHirschsprung's diseasemTORmiR-199a-3p

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