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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
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4C Analysis of EBV-Host DNA Interactome.
Kyoung-Dong Kim1, Paul M Lieberman2
1Department of Systems Biotechnology, Chung-Ang University, Anseong, South Korea. kdkim0122@cau.ac.kr.
Methods in Molecular Biology (Clifton, N.J.)
|December 19, 2022
Summary
This study details a 4C-seq protocol to map Epstein-Barr virus (EBV) episomes in host cells. Understanding EBV
Area of Science:
- Molecular Biology
- Virology
- Genomics
Background:
- Epstein-Barr virus (EBV) persists as episomes in infected cells, altering host gene expression.
- Identifying EBV's chromosomal attachment sites is crucial for understanding its regulatory mechanisms.
Purpose of the Study:
- To present a detailed protocol for 4C-seq analysis to identify EBV episome tethering sites.
- To provide a method applicable to various cell types for studying viral-host interactions.
Main Methods:
- The protocol involves cell fixation, 4C-DNA construction, and sequencing library preparation.
- Experiments were conducted using EBV-positive Burkitt's lymphoma cells.
Main Results:
- The developed 4C-seq method successfully identified target loci associated with EBV episomes.
- This technique allows for the mapping of viral episome interactions within the host genome.
Conclusions:
- The 4C-seq protocol is a valuable tool for investigating EBV episome integration and regulation.
- This method facilitates research into viral-host chromosome interactions across different cell types.
