Assessing dual-energy X-ray absorptiometry prediction of intramuscular fat content in beef longissimus steaks

This study assessed the capability of dual-energy X-ray absorptiometry (DEXA) to predict intramuscular fat (IMF) content of beef longissimus steaks against chemical IMF as the gold standard. DEXA performance of fat% prediction was assessed using a leave-one-out cross validation method among Angus and Nellore steaks, which generated a chemical fat% range of 14.05-36.82% and 2.46-7.84%, respectively, and using pooled data. There was a significant positive association between DEXA predicted fat and chemical fat content. However, higher precision was found for pooled data (R² = 0.95, RMSECV = 1.95) and Angus (R² = 0.75, RMSECV = 2.39) than Nellore (R² = 0.15, RMSECV = 1.22) group. Accuracy also had the same response with average slope values close to 1 for pooled data and Angus and a lower value (0.42) for Nellore group. DEXA precisely predicts IMF content across a wide range of fat content. However, its precision and accuracy of prediction within low-fat content samples are lower than in high-fat content.