Transcriptome-wide profiling of mRNA N⁶-methyladenosine modification in rice panicles and flag leaves

N⁶-methyladenosine (m⁶A) modification is essential for plant growth and development. Exploring m⁶A methylation patterns in rice tissues is fundamental to understanding the regulatory effects of this modification. Here, we profiled the transcriptome-wide m⁶A landscapes of rice panicles at the booting stage (PB) and flowering stage (PF), and of flag leaves at the flowering stage (LF). The global m⁶A level differed significantly among the three tissues and was closely associated with the expression of writer and eraser genes. The methylated gene ratio was higher in the flag leaves than in the panicles. Compared with commonly methylated genes, tissue-specific methylated genes showed lower levels of both m⁶A modification and expression, and a preference for m⁶A deposition in the coding sequence region. The m⁶A profiles of the two organs had more distinct differences than the profiles of the same organ at different stages. A negative correlation between m⁶A levels and gene expression was observed in PF vs. PB but not in PF vs. LF, indicting the complicated regulatory effect of m⁶A on gene expression. The distinct expression patterns of m⁶A reader genes in different tissues indicate that readers may affect gene stability through binding. Overall, our findings demonstrated that m⁶A modification influences tissue function by regulating gene expression. Our findings provide valuable insights on the regulation and biological functions of m⁶A modifications in rice.