Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Orphaned Innovation: Cost, Structural Barriers, and Global System Shifts Prompting Reimbursement Reform in Endoscopic Spine Surgery-Insights From 1.2 Million Cases.

International journal of spine surgery·2026
Same author

Physical activity and patient-reported outcomes after decompressive endoscopic lumbar spine surgery.

Journal of neurosurgery. Spine·2026
Same author

The first week matters: App-based PROM trajectories and follow-up retention after endoscopic lumbar surgery.

Brain & spine·2026
Same author

Intraoperative Neuromonitoring Events in Transforaminal Endoscopy Using the Trans-Superior Articular Process Approach.

Operative neurosurgery (Hagerstown, Md.)·2026
Same author

Transforaminal endoscopic lumbar discectomy versus interlaminar endoscopic lumbar discectomy for lumbar disc herniations: a prospective multicenter study of recovery trajectories using high-frequency patient-reported outcome measures.

The spine journal : official journal of the North American Spine Society·2026
Same author

Disruptive technologies in spine surgery: current trends, outcomes, and ethical implications.

Journal of neurosurgery. Spine·2026

Related Experiment Video

Updated: Aug 16, 2025

Imaging and Quantification of the Hepatic Vasculature of Mice Using Ultrafast Doppler Ultrasound
07:03

Imaging and Quantification of the Hepatic Vasculature of Mice Using Ultrafast Doppler Ultrasound

Published on: July 19, 2024

966

Quantitative tissue perfusion imaging using nonlinear ultrasound localization microscopy.

Jennifer N Harmon1, Zin Z Khaing1, Jeffrey E Hyde1

  • 1Department of Neurological Surgery, University of Washington, Seattle, WA, 98105, USA.

Scientific Reports
|December 19, 2022
PubMed
Summary
This summary is machine-generated.

Nonlinear ultrasound localization microscopy (ULM) images microbubbles regardless of velocity, enabling visualization of the entire vascular tree. This new method quantifies tissue perfusion, crucial for understanding blood flow quality.

More Related Videos

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
06:02

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

4.0K
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

16.3K

Related Experiment Videos

Last Updated: Aug 16, 2025

Imaging and Quantification of the Hepatic Vasculature of Mice Using Ultrafast Doppler Ultrasound
07:03

Imaging and Quantification of the Hepatic Vasculature of Mice Using Ultrafast Doppler Ultrasound

Published on: July 19, 2024

966
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
06:02

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

4.0K
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

16.3K

Area of Science:

  • Biomedical Imaging
  • Ultrasound Technology
  • Vascular Biology

Background:

  • Conventional ultrasound localization microscopy (ULM) faces limitations in imaging microvascular blood flow due to velocity-based signal filtering.
  • Existing methods struggle to visualize the complete vascular tree and assess tissue perfusion at the microcirculatory level.

Purpose of the Study:

  • To develop and validate a novel nonlinear ultrasound localization microscopy (ULM) technique for velocity-independent microbubble signal segmentation.
  • To enable comprehensive imaging of the entire vascular tree and quantitative assessment of microcirculatory flow quality.

Main Methods:

  • Developed nonlinear ULM by integrating nonlinear pulsing sequences with plane-wave imaging.
  • Utilized bubble localization and inter-frame tracking for super-resolved imaging.
  • Derived quantitative features from bubble tracks to assess microcirculatory flow quality.

Main Results:

  • Nonlinear ULM demonstrated improved resolution of smaller vasculature compared to conventional ULM in a rat spinal cord model.
  • Both nonlinear ULM and histology showed reduced in-plane vessel length post-injury, indicating impaired blood flow.
  • The study successfully visualized and quantified tissue perfusion, a key aspect of blood flow.

Conclusions:

  • Nonlinear ULM overcomes the velocity-dependent limitations of conventional ULM, allowing for comprehensive vascular imaging.
  • This technique uniquely enables the investigation and quantification of tissue perfusion.
  • Nonlinear ULM holds significant potential for advancing the study of microcirculatory disorders and therapeutic efficacy.