TEAD1 Silencing Regulates Cell Proliferation and Resistance to 5-Fluorouracil in Cutaneous Squamous Cell Carcinoma
Ziyang Wang1, Meng Liu1, Hao Lei1
1Department of Dermatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.
Purpose:
Cutaneous squamous cell carcinoma (cSCC) is a skin malignant tumor account for approximately one-third of all nonmelanoma skin cancers. Studies have shown that TEA domain transcription factor 1 (TEAD1) is discovered to be involved in the pathogenesis of some human cancers, but to our knowledge its role in cSCC has not been reported.
Patients And Methods:
Samples from 16 cSCC patients and 27 healthy individuals were obtained for immunohistochemical staining of TEAD1. The expressions of TEAD1 in SCL-1, HSC-1 cells compared with the primary neonatal human epithelial keratinocytes were detected by Western blot and RT-qPCR. Proliferation and cell cycle of TEAD1 knockdown in cSCC cell lines were examined by MTT and flow cytometry analysis. Annexin V/PI and JC-1 staining were used to determine the cell apoptosis.
Results:
The expression of TEAD1 decreased significantly in cSCC compared to its expression in normal skin tissues and cell lines. Down-regulation of TEAD1 in cSCC cell lines promoted cell growth via regulation of the G2/M progression. Additionally, silence of TEAD1 also protected cells against 5-Fluorouracil-induced apoptosis and decreased the expression of apoptosis-related protein (p53).
Conclusion:
Our results suggested that TEAD1 expression is down-regulated and functioned as a tumor suppressor in cSCC and that it may serve as a biomarker or therapeutic target of cSCC.
Insights
TEA domain transcription factor 1 (TEAD1) is down-regulated in cutaneous squamous cell carcinoma (cSCC), acting as a tumor suppressor. This finding suggests TEAD1 may be a potential biomarker or therapeutic target for cSCC.
Area of Science:
- Oncology
- Dermatology
- Molecular Biology
Background:
- Cutaneous squamous cell carcinoma (cSCC) is a common nonmelanoma skin cancer.
- TEA domain transcription factor 1 (TEAD1) is implicated in various human cancers.
- The role of TEAD1 in cSCC pathogenesis was previously unreported.
Purpose of the Study:
- To investigate the expression and function of TEAD1 in cSCC.
- To determine if TEAD1 plays a role in cSCC development.
- To explore TEAD1 as a potential biomarker or therapeutic target for cSCC.
Main Methods:
- Immunohistochemical staining of TEAD1 in cSCC tissues and normal skin.
- Western blot and RT-qPCR to assess TEAD1 expression in cell lines.
- MTT assays, flow cytometry, Annexin V/PI, and JC-1 staining to evaluate proliferation, cell cycle, and apoptosis in TEAD1-knockdown cSCC cells.
Main Results:
- TEAD1 expression was significantly decreased in cSCC tissues and cell lines compared to normal controls.
- Down-regulation of TEAD1 promoted cSCC cell proliferation by affecting G2/M cell cycle progression.
- TEAD1 knockdown reduced apoptosis and decreased p53 expression in response to 5-Fluorouracil treatment.
Conclusions:
- TEAD1 functions as a tumor suppressor in cSCC.
- Down-regulation of TEAD1 is associated with cSCC progression.
- TEAD1 represents a potential biomarker and therapeutic target for cSCC.
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