Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

751
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
751
Aliasing01:18

Aliasing

192
Accurate signal sampling and reconstruction are crucial in various signal-processing applications. A time-domain signal's spectrum can be revealed using its Fourier transform. When this signal is sampled at a specific frequency, it results in multiple scaled replicas of the original spectrum in the frequency domain. The spacing of these replicas is determined by the sampling frequency.
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original...
192
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of a child with Oculocutaneous albinism due to compound heterozygous variants of OCA2 gene].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same author

Widefield pump-probe microscopy with coherent background subtraction by angle-compensated temporal shearing.

Optics express·2026
Same author

TB-DLossNet: Fine-Grained Segmentation of Tea Leaf Diseases Based on Semantic-Visual Fusion.

Plants (Basel, Switzerland)·2026
Same author

The therapeutic potential of vitamins as nutrients in food for anti-inflammatory and anti-oxidative stress in liver fibrosis diseases.

Frontiers in nutrition·2026
Same author

Subthreshold membrane depolarization powerfully engages intracellular calcium dynamics in the brain.

bioRxiv : the preprint server for biology·2026
Same author

Implications of temporal sampling in voltage imaging microscopy.

ArXiv·2026

Related Experiment Video

Updated: Aug 16, 2025

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
05:24

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

Published on: November 11, 2010

23.9K

Correcting sampling bias in speckle contrast imaging.

Shuqi Zheng, Jerome Mertz

    Optics Letters
    |December 20, 2022
    PubMed
    Summary

    This study introduces a method to accurately measure speckle contrast in laser speckle contrast imaging (LSCI) by correcting for bias caused by limited speckle grains. This improves the estimation of speckle decorrelation time in various imaging conditions.

    Area of Science:

    • Biomedical Optics
    • Image Analysis
    • Laser Physics

    Background:

    • Laser Speckle Contrast Imaging (LSCI) is widely used for blood flow monitoring.
    • Estimating speckle contrast typically uses small analysis windows, leading to bias due to insufficient speckle statistics.
    • This bias affects the accuracy of derived parameters like speckle decorrelation time.

    Purpose of the Study:

    • To develop and validate a method for accurate speckle contrast estimation in LSCI.
    • To correct for the systematic bias introduced by limited speckle grains (NS).
    • To enable more precise measurement of speckle decorrelation time without complex data fitting.

    Main Methods:

    • Developed an approach to determine the number of independent speckle grains (NS).

    More Related Videos

    Sample Drift Correction Following 4D Confocal Time-lapse Imaging
    10:04

    Sample Drift Correction Following 4D Confocal Time-lapse Imaging

    Published on: April 12, 2014

    16.5K
    High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
    08:18

    High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

    Published on: June 16, 2020

    7.5K

    Related Experiment Videos

    Last Updated: Aug 16, 2025

    How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
    05:24

    How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow

    Published on: November 11, 2010

    23.9K
    Sample Drift Correction Following 4D Confocal Time-lapse Imaging
    10:04

    Sample Drift Correction Following 4D Confocal Time-lapse Imaging

    Published on: April 12, 2014

    16.5K
    High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
    08:18

    High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

    Published on: June 16, 2020

    7.5K
  • Implemented a bias correction method based on the determined NS.
  • Validated the method using both ergodic and non-ergodic measurements, including in vivo mouse brain imaging.

    • Main Results:

    • Successfully determined NS in LSCI data.
    • Demonstrated significant correction of the systematic bias in speckle contrast estimation.
    • Achieved more accurate speckle decorrelation time measurements.

    Conclusions:

    • The proposed method provides a robust way to improve LSCI accuracy.
    • Accurate NS determination and bias correction are crucial for reliable speckle decorrelation time estimation.
    • This technique is applicable to diverse LSCI scenarios, including in vivo biological imaging.