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Related Concept Videos

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Skin Diseases and Disorders

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Skin is the first line of defense and encounters a variety of microbes. Some pathogenic strains are often the cause of a broad range of infections of the skin and other body systems. These conditions can affect people of all ages and may have different causes, including genetic factors, infections, autoimmune reactions, environmental factors, and lifestyle choices.
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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Surface Membrane Barriers01:18

Surface Membrane Barriers

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The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
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Defense Mechanism Against Infection01:26

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Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
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Tissue Membranes01:27

Tissue Membranes

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A tissue membrane is a thin layer of cells that covers the outside of the body, the organs, internal passageways that lead to the exterior of the body, and the lining of the moveable joint cavities. There are two basic types of tissue membranes— connective tissue and epithelial membranes.
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Factors Affecting the Risk of Infection01:26

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The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
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Protocol to Create Chronic Wounds in Diabetic Mice
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Cutaneous Surgical Wounds Have Distinct Microbiomes from Intact Skin.

Sameer Gupta1, Alexandra J Poret2,3, David Hashemi1

  • 1Department of Dermatology, MGH, Boston, Massachusetts, USA.

Microbiology Spectrum
|December 21, 2022
PubMed
Summary
This summary is machine-generated.

Skin commensals may protect against surgical site infections. This study profiled wound microbiomes after skin cancer surgery, finding enriched Corynebacterium and Staphylococcus aureus in healing wounds, suggesting a protective role against pathogens.

Keywords:
16S RNACorynebacteriumhuman microbiomeinfectious diseaseskin microbiome

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Area of Science:

  • Microbiology
  • Dermatology
  • Surgical Infections

Background:

  • Cutaneous surgical procedures carry a risk of infection, yet the role of skin commensals in preventing this is unclear.
  • The microbiome of surgical skin wounds has not been extensively studied using culture-independent methods.
  • Understanding the wound microbiome is crucial for developing strategies to mitigate infection risk.

Purpose of the Study:

  • To characterize the microbiome of surgical skin wounds in patients healing without infection.
  • To compare the wound microbiome with the microbiome of intact skin from the same patients.
  • To identify bacterial species that may play a role in colonization resistance and normal wound healing.

Main Methods:

  • Culture-independent profiling of the wound microbiome in 53 patients undergoing skin cancer surgery.
  • Comparison of bacterial species abundance in wounds versus adjacent intact skin one week post-surgery.
  • Analysis of microbial composition in relation to wound healing outcomes.

Main Results:

  • Cutibacterium acnes abundance was reduced 5-fold in wounds compared to intact skin.
  • Staphylococcus aureus, a pathogen, was enriched 6.4-fold in clinically noninfected wounds.
  • Corynebacterium species dominated the wound microbiome (37%), suggesting a protective role.

Conclusions:

  • The wound microbiome differs significantly from intact skin microbiome post-surgery.
  • Enrichment of Corynebacterium in healing wounds suggests potential colonization resistance against pathogens.
  • Further research into Corynebacterium's role could lead to novel strategies for preventing surgical wound infections.