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Updated: Aug 16, 2025
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
Published on: August 4, 2017
A modified hydrogel production protocol to decrease cellular content
Gabriela Catão Diniz Braga1, Cristina Pires Camargo2, Martin Conrad Harmsen3
1Bachelor. Universidade de São Paulo - Discipline of Plastic Surgery, Microsurgery and Plastic Surgery Laboratory - School of Medicine - São Paulo (SP), Brazil.
Purpose:
To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix.
Methods:
We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation.
Results:
After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days.
Conclusions:
The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.
