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Evaluation of two fungal exopolysaccharides as potential biomaterials for wound healing applications
Masoud Hamidi1,2, Oseweuba Valentine Okoro1, Khodabakhsh Rashidi3
1Université Libre de Bruxelles (ULB), École Polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050, Brussels, Belgium.
Abstract:
Microbial exopolysaccharides (EPSs) are mostly produced by bacteria and fungi and have potential use in the production of biomedical products such as nutraceuticals and in tissue engineering applications. The present study investigated the in vitro biological activities and in vivo wound healing effects of EPSs produced from a Sclerotium-forming fungus (Sclerotium glucanicum DSM 2159) and a yeast (Rhodosporidium babjevae), denoted as scleroglucan (Scl) and EPS-R, respectively. EPS yields of 0.9 ± 0.07 g/L and 1.11 ± 0.4 g/L were obtained from S. glucanicum and R. babjevae, respectively. The physicochemical properties of the EPSs were characterized using infrared spectroscopy and scanning electron microscopy. Further investigations of the biological properties showed that both EPSs were cytocompatible toward the human fibroblast cell line and demonstrated hemocompatibility. Favorable wound healing capacities of the EPSs (10 mg/mL) were also established via in vivo tests. The present study therefore showed that the EPSs produced by S. glucanicum and R. babjevae have the potential use as biocompatible components for the promotion of dermal wound healing.
Insights
Microbial exopolysaccharides (EPSs) from fungus and yeast show promise for biomedical applications. These EPSs demonstrated biocompatibility and enhanced wound healing in vivo, suggesting potential for dermal applications.
Area of Science:
- Microbiology and Biotechnology
- Biomaterials Science
Background:
- Microbial exopolysaccharides (EPSs) are versatile biopolymers with potential biomedical applications.
- Fungal and yeast-derived EPSs are increasingly explored for their unique properties.
Purpose of the Study:
- To investigate the in vitro biological activities and in vivo wound healing effects of EPSs from Sclerotium glucanicum and Rhodosporidium babjevae.
- To characterize the physicochemical and biological properties of scleroglucan (Scl) and EPS-R.
Main Methods:
- EPSs were extracted from Sclerotium glucanicum and Rhodosporidium babjevae.
- Physicochemical properties were analyzed using infrared spectroscopy and scanning electron microscopy.
- In vitro cytocompatibility and hemocompatibility were assessed, alongside in vivo wound healing tests.
Main Results:
- EPS yields of 0.9 g/L and 1.11 g/L were obtained, respectively.
- Both EPSs exhibited cytocompatibility with human fibroblasts and hemocompatibility.
- Significant wound healing capacities were observed in vivo at 10 mg/mL concentration.
Conclusions:
- EPSs from S. glucanicum and R. babjevae are biocompatible.
- These microbial EPSs show potential for promoting dermal wound healing.
- Further research into these EPSs could lead to novel biomaterials for regenerative medicine.
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