A Ratiometric Probe Based on Carbon Dots and Calcein & Eu³⁺ for the Fluorescent Detection of Sodium Tripolyphosphate

Sodium tripolyphosphate, a food additive, is applied broadly in food industry. However, excessive accumulation of sodium tripolyphosphate can result in electrolyte abnormality of the body. Therefore, it is of great importance to investigate an effective method for the detection of sodium tripolyphosphate. In this work, nitrogen-doped carbon dots (NCDs) with constant fluorescence were fabricated using a domestic microwave oven. A ratiometric fluorescent probe was constructed in which NCDs were as internal standard, calcein & Eu³⁺ were as the detection signal. The fluorescence of calcein at 515 nm was quenched by Eu³⁺, whereas the emission peak of NCDs at 446 nm was almost unchanged. Additionally, the fluorescence of calcein was recovered because of the strong interaction of sodium tripolyphosphate and Eu³⁺. The linear range for sodium tripolyphosphate was 0.5-6 µmol/L with detection limit of 0.12 µmol/L. Furthermore, the ratiometric fluorescent probe was applied for sodium tripolyphosphate detection in real milk samples.