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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Production of Haploid Zebrafish Embryos by In Vitro Fertilization
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Large-scale forward genetic screening of zebrafish affecting thyroid development.

Jia-Ping Wan1, Zheng Wang1, Cao-Xu Zhang1

  • 1Department of Molecular Diagnostics & Endocrinology, The Core Laboratory in Medical Center of Clinical Research, Shanghai Ninth People's Hospital, State Key Laboratory of Medical Genomics, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.

Biochemical and Biophysical Research Communications
|December 21, 2022
PubMed
Summary

Researchers used zebrafish to identify genes controlling thyroid development. This forward mutagenesis screen identified 112 mutant families, offering insights into thyroid function and developmental disorders.

Keywords:
Congenital hypothyroidismENUForward mutagenesis screenThyroid developmentZebrafish

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Area of Science:

  • Developmental Biology
  • Genetics
  • Endocrinology

Background:

  • Thyroid follicular cells develop from the foregut endoderm.
  • Understanding the genes and signaling pathways governing thyroid development is essential for addressing developmental disorders and adult diseases.

Purpose of the Study:

  • To identify genes regulating thyroid follicular cell development and function using a forward mutagenesis screen in zebrafish.
  • To characterize novel mutations affecting thyroid development.

Main Methods:

  • An N-ethyl-N-nitrosourea (ENU) chemical mutagenesis screen was performed in zebrafish.
  • 1606 F2 families were analyzed, and embryos at 5 days post-fertilization (dpf) were examined using whole-mount in situ hybridization with thyroid (thyroglobulin) and pituitary (thyroid stimulating hormone) markers.
  • Mutant families exhibiting thyroid dysfunction were categorized based on phenotypic characteristics.

Main Results:

  • Out of 1606 F2 families, 112 families displayed thyroid dysfunction while maintaining normal developmental stages.
  • These 112 mutant families were classified into three distinct phenotypic groups.
  • The screen successfully identified mutations affecting thyroid development and function.

Conclusions:

  • The zebrafish mutagenesis screen is effective for identifying genes involved in thyroid development.
  • The identified mutants provide valuable models for further investigation into the molecular mechanisms of thyroid development and function in vertebrates.
  • These findings contribute to understanding both normal development and disease pathologies related to the thyroid gland.