Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human macrophage development: a morphometric study.

R J Sokol1, G Hudson, N T James

  • 1Department of Haematology, University of Sheffield, England.

Journal of Anatomy
|April 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating a psychological model of mental conditions and coping during the COVID-19 pandemic driven by participatory methods.

Social psychiatry and psychiatric epidemiology·2022
Same author

Neuropathological and biochemical investigation of Hereditary Ferritinopathy cases with ferritin light chain mutation: Prominent protein aggregation in the absence of major mitochondrial or oxidative stress.

Neuropathology and applied neurobiology·2020
Same author

Communication with young people in paediatric and adult endocrine consultations: an intervention development and feasibility study.

BMC endocrine disorders·2017
Same author

Exome sequencing in dementia with Lewy bodies.

Translational psychiatry·2016
Same author

Extraction Time of Kidneys From Deceased Donors and Impact on Outcomes.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

Loss of pediatric kidney grafts during the "high-risk age window": insights from pediatric liver and simultaneous liver-kidney recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015

Human macrophages significantly increase in size and complexity during six days in culture. This study provides key morphometric data for understanding macrophage development and potential impairments in diseases like cancer.

Area of Science:

  • Cell Biology
  • Immunology
  • Morphology

Background:

  • Macrophages are crucial immune cells derived from blood monocytes.
  • Understanding macrophage development is vital for immunology and disease research.
  • Previous studies lacked detailed morphometric data on in vitro macrophage development.

Purpose of the Study:

  • To quantitatively analyze the morphometric changes during human monocyte-derived macrophage development in culture.
  • To establish baseline data for normal macrophage development.
  • To provide a foundation for investigating impaired macrophage development in diseases.

Main Methods:

  • Utilized suspension cultures for normal human blood monocytes over six days.
  • Performed morphometric measurements on electron micrographs.

Related Experiment Videos

  • Applied logarithmic transformation and two-way analysis of variance for statistical analysis.

    • Main Results:

    • Observed a 3-4 fold increase in whole cell and cytoplasmic volumes.
    • Cell surface area more than doubled due to increased volume and irregularity.
    • Mitochondrial volume significantly increased, with constant cytoplasmic volume fraction.

    Conclusions:

    • Established comprehensive morphometric data for human macrophage development in vitro.
    • The findings provide a baseline for future research into macrophage dysfunction in diseases.
    • The experimental system is suitable for studying factors affecting macrophage development in malignant conditions.