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Updated: Aug 16, 2025
Use of Human Perivascular Stem Cells for Bone Regeneration
Published on: May 25, 2012
Use of Melatonin/Decorticotomy and Autogenous Bone Graft in Induced 1-Wall Defect
Hala H Hazzaa1, Mai S Attia2, Marwa A M El Shiekh3
1Professor of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt; Professor of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Dakahlia, Egypt.
Purpose:
This study was designed to investigate the effect of intramarrow penetration (IMP) and 1% melatonin (MLN) gel on the remodelling process of autogenous bone graft (ABG) in an induced 1-osseous wall defect model.
Methods:
Sixty-four intrabony induced mandibular defects were created on the distal side of premolars-P1, P2, P3, and P4 (on each side)-in 8 beagle dogs. A ligature-induced periodontitis was initiated in each defect. Defects were then divided into 4 equal groups. Group I was treated with open-flap debridement (OFD) alone, group II was treated with OFD/ABG, group III was treated with OFD/IMP/ABG, and group IV was treated with OFD/ABG/IMP/1% MLN gel. The study parameters were bone fill, histologic analysis, and immunohistochemical evaluation of endothelial nitric oxide synthase (eNOS) expression at 2-week (2W) and 8-week (8W) time intervals.
Results:
At 8W, significant differences were revealed amongst all groups regarding the amount of bone fill and eNOS expressions (P < .001). Bone fill percentages were 55.5%, 22.3%, 16.8%, and 0% in groups IV, III, II, and I, respectively. eNOS expressions were 1.68 ± 0.06, 8.43 ± 0.04, 16.80 ± 0.17, and 1.97 ± 0.07 in groups IV, III, II, and I, respectively. The favourable results were in line with group IV.
Conclusions:
According to these preliminary results, defects treated by ABG augmented with IMP and 1% MLN gel revealed a greater amount of bone fill and reduced eNOS expression. This combination is therefore highly suggested as an adjunct to ABG.
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