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In vitro elution of amikacin from four hydrogel preparations: A pilot study
Natasha M Hottmann1, Katherine Raines2, Madeline Droog2
1Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Objective:
To determine in vitro elution of amikacin from poloxamer 407 NF, 1% carboxymethylcellulose (CMC), 3% CMC, 5% CMC, and control (sterile water).
Study Design:
Descriptive in vitro.
Sample Population:
Triplicate samples from each experimental group.
Methods:
Amikacin solution was prepared in poloxamer 407 NF, 1% CMC, 3% CMC, 5% CMC, or sterile water. Then, 1 ml of phosphate-buffered saline (PBS) was added to each of three aliquots per base and the samples were incubated at 37°C. PBS was removed and replaced at 1, 4, 8, 12, 24, 48, 72, 96, 120, 144, 168, 192, 216, and 240 hours and amikacin concentration was measured.
Results:
The highest median concentration of amikacin in the eluent of poloxamer 407 NF, 3% CMC, and 5% CMC was observed at 48 hours: 3300, 3030, and 2190 μg/ml, respectively. The highest median concentration of amikacin in the eluent of 1% CMC and sterile water were observed at 1 hour: 13300 and 15600 μg/ml, respectively. Median eluent concentration of amikacin exceeded 2000 μg/ml (the reported minimum inhibitory concentration [MIC] of certain biofilm-producing methicillin-resistant Staphylococcus pseudintermedius) from 24 to 96 hours for poloxamer 407 NF, 24-72 hours for 3% CMC, 48-72 hours for 5% CMC, 1-4 hours for 1% CMC, and 1-4 hours for sterile water.
Conclusion:
Amikacin elution from tested substances reached or exceeded target MIC during the 240 hours tested.
Clinical Significance:
Hydrogel-amikacin solutions may be useful topical treatment options for some infected wounds. In vivo safety and efficacy should be evaluated.
