Identification of the molecular mechanism and candidate markers for diabetic nephropathy

Diabetic nephropathy (DN) is one of the most common complications in diabetic patients. New strategies are needed to delay the occurrence and development of this pathology.

Differentially expressed genes (DEGs) in glomeruli and renal tubules were identified using the GSE30122 dataset, and a co-expression network was constructed to identify the hub genes of modules. The biological function and signaling pathway of the module genes were also analyzed. In addition, the expression of 24 immune cells and the area under the receiver operating characteristic (ROC) curve (AUC) values of the hub genes were also calculated.

A total of 1,778 DEGs were isolated from glomeruli and 1,996 DEGs were isolated from renal tubules. Nine modules and their hub genes were identified using the co-expression network. Enrichment analysis showed that the module genes were mainly enriched in immune inflammation and oxidative stress. The expressions of B cells, activated dendritic cell, and T cells in the glomeruli and renal tubules of DN patients were higher than those in the controls, and the correlation between these immune cells was the strongest. Collagen type I alpha 2 chain (COL1A2), the hub gene of the brown module, had the highest AUC values and may have a better clinical diagnostic ability.

In conclusion, the module genes and related biological functions and signaling pathways found in this study can deepen our understanding of the molecular mechanism of DN progression. COL1A2 may be a potential biomarker for DN.