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Updated: Jun 20, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Published on: May 8, 2013

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Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Joo-Kyung Kim1, Hanool Yun2, Chang-Hun Yeom2

  • 1Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University Seoul 08826 South Korea.

RSC Advances
|December 22, 2022
PubMed

Insights

Rapidly detect Staphylococcus aureus and Pseudomonas aeruginosa using flow cytometry. This method identifies pathogens in under an hour, crucial for food safety and infection control.

Area of Science:

  • Microbiology
  • Immunology
  • Analytical Chemistry

Background:

  • Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are significant foodborne pathogens causing poisoning and persistent infections.
  • Early detection of these bacteria is critical to prevent widespread contamination and mitigate health risks.

Purpose of the Study:

  • To establish a rapid flow cytometry (FCM)-based detection method for S. aureus and P. aeruginosa.
  • To enable early identification of these major pathogens in various sample types.

Main Methods:

  • Development of an FCM assay utilizing fluorescent antibodies specific to S. aureus and P. aeruginosa.
  • Quantification of bacterial populations in fluid samples through rapid cell acquisition.

Main Results:

  • The FCM method achieved a limit of detection in the order of 10^3 to 10^5 CFU for both pathogens.
  • Detection and analysis were completed within one hour, demonstrating significant speed.

Conclusions:

  • The developed FCM method provides a rapid and efficient means for detecting S. aureus and P. aeruginosa.
  • This high-throughput technique is applicable to pathogen analysis in food, environmental, and clinical samples.

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