Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cognitive engagement induces area-specific fingerprints of dopamine, acetylcholine, serotonin, glutamate and GABA in prefrontal cortex and striatum.

bioRxiv : the preprint server for biology·2026
Same author

Nonexhaustive microextraction as a step toward more sustainable chemical analysis in the field and the clinic.

Nature protocols·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same author

Advances in LC-MS/MS methods and sample preparation strategies for therapeutic drug monitoring of immunosuppressive drugs.

Journal of chromatography. A·2026
Same author

Preoperative untargeted SPME-LC/HRMS-based metabolomic profiling in cardiac surgical patients identified prognostic biomarkers for postoperative outcomes.

Analytica chimica acta·2025

Related Experiment Video

Updated: Aug 16, 2025

Synthesis and Characterization of Multi-Modal Phase-Change Porphyrin Droplets
07:59

Synthesis and Characterization of Multi-Modal Phase-Change Porphyrin Droplets

Published on: October 15, 2021

3.7K

Standard Water Generating Vials for Lipophilic Compounds.

Demet Dincel1,2, Hernando Rosales-Solano1, Shakiba Zeinali1

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.

Analytical Chemistry
|December 22, 2022
PubMed
Summary

A novel in-vial system generates stable aqueous standards for hydrophobic compounds. This method ensures consistent analyte concentrations, overcoming solubility challenges for reliable chemical analysis and quality control.

More Related Videos

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
09:09

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

2.2K
VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders
08:57

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders

Published on: August 3, 2016

11.0K

Related Experiment Videos

Last Updated: Aug 16, 2025

Synthesis and Characterization of Multi-Modal Phase-Change Porphyrin Droplets
07:59

Synthesis and Characterization of Multi-Modal Phase-Change Porphyrin Droplets

Published on: October 15, 2021

3.7K
Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis
09:09

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

2.2K
VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders
08:57

VacuSIP, an Improved InEx Method for In Situ Measurement of Particulate and Dissolved Compounds Processed by Active Suspension Feeders

Published on: August 3, 2016

11.0K

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Biochemistry

Background:

  • Non-polar compounds exhibit poor solubility in aqueous media, leading to adsorption on surfaces and unstable concentrations.
  • Accurate quantification of hydrophobic analytes in water is crucial for various scientific disciplines.
  • Existing methods struggle to maintain stable concentrations of hydrophobic compounds in aqueous solutions.

Purpose of the Study:

  • To develop an in-vial standard water generating system for hydrophobic compounds.
  • To enable the preparation of stable mixtures of hydrophobic compounds in aquatic environments.
  • To address the challenge of unstable sample concentrations caused by poor solubility.

Main Methods:

  • Introduction of an in-vial system with an aqueous solution and a polydimethylsiloxane thin film spiked with target compounds.
  • Achieving equilibrium between the thin film and aqueous solution to attain stable analyte concentrations.
  • Utilizing endocannabinoids and phospholipids as model hydrophobic compounds for system validation.

Main Results:

  • The developed system maintains stable concentrations of hydrophobic compounds in water for multiple days.
  • Analyte release from the thin film compensates for losses during solid-phase microextraction (SPME) fiber extractions.
  • Demonstrated stability and reproducibility of the standard water generating system.

Conclusions:

  • The in-vial system is suitable for repeatable generation of non-polar standards for routine analysis and quality control.
  • The system provides stable and reproducible measurements of free concentrations for accurate quantification.
  • This approach overcomes limitations associated with hydrophobic compound solubility in aqueous analysis.