Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Driving change in Nova Scotia: The lasting impact of Public Health Mobile Units beyond COVID-19.

Canadian journal of public health = Revue canadienne de sante publique·2026

Same author

Bioarcheological Perspectives on the Timing of Adolescence in Rural Avar-Age Austria, 7th-9th Centuries ce.

American journal of biological anthropology·2025

Same author

Implementation of a Malignant Hematology Education Intervention and its Impact on Hematology Nurse Practitioner Knowledge and Self-Efficacy to Practice.

Journal of the advanced practitioner in oncology·2025

Same author

The 2022 and 2023 Emergency Medicine Residency Match: A Cautionary Tale.

Cureus·2023

Same author

Improving Hospital Infant Safe Sleep Compliance by Using Safety Prevention Bundle Methodology.

Pediatrics·2021

Same author

The role of Raman spectroscopy in biopharmaceuticals from development to manufacturing.

Analytical and bioanalytical chemistry·2021

Same journal

EXPRESS: Deterministic Compressed Sensing in Time-Domain Spectroscopy.

Applied spectroscopy·2026

Same journal

EXPRESS: Multi-Parameter Wavelength Characterization of Array Spectrometers Under Near-Limit Sampling Conditions.

Applied spectroscopy·2026

Same journal

EXPRESS: A Validated Reference Database for Twentieth-Century Cd-Based Pigments: Integrated Structural and Compositional Characterization.

Applied spectroscopy·2026

Same journal

EXPRESS: Two-Trace Two-Dimensional (2T2D-COS) in the Analysis of Brain Tissue Sample Preparation Method.

Applied spectroscopy·2026

Same journal

EXPRESS: Simplified Protocol for Analyzing Polarization Properties of Scanning Tunneling Microscope (STM) Light Emission Spectra at an Oblique Angle.

Applied spectroscopy·2026

Same journal

EXPRESS: Monitoring a Polyurethane Synthesis by Fiber-Coupled Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Multivariate Curve Resolution-Alternating Least Squares.

Applied spectroscopy·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 16, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Direct Measurement of Chocolate Components Using Dispersive Raman Spectroscopy at 1000 nm Excitation.

Karen Esmonde-White¹, Mary Lewis¹, Ian R Lewis¹

¹Endress+Hauser Optical Analysis, Ann Arbor, MI, USA.

Applied Spectroscopy

|December 22, 2022

Summary

This summary is machine-generated.

New Raman spectroscopy using 1000 nm excitation significantly reduces fluorescence in dark and milk chocolates. This advancement enables non-destructive analysis of chocolate composition and structure, crucial for quality control in confectionary processing.

Keywords:

Raman spectroscopy chocolate cocoa butter polymorphism sucrose

More Related Videos

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

Related Experiment Videos

Last Updated: Aug 16, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems

Published on: April 28, 2016

Area of Science:

Food Science
Analytical Chemistry
Spectroscopy

Background:

Chocolate production involves critical steps like fermentation, roasting, grinding, and controlled crystallization to achieve desired texture and polymorph V.
Raman spectroscopy offers non-contact, non-destructive analysis of chemical composition and molecular structure.
Visible and near-infrared excitation (532-785 nm) in Raman spectroscopy is hindered by overwhelming fluorescence in dark and milk chocolates.

Purpose of the Study:

To investigate the potential of 1000 nm excitation Raman spectroscopy to overcome fluorescence limitations in dark and milk chocolates.
To assess the feasibility of using 1000 nm excitation for direct, non-destructive analysis of various chocolate types.

Main Methods:

Dispersive Raman spectroscopy utilizing 1000 nm excitation was employed.
Samples analyzed included white chocolate, milk chocolate, dark chocolate, and cocoa nibs.
The study focused on reducing sample fluorescence for improved spectral acquisition.

Main Results:

1000 nm excitation effectively reduced fluorescence in dark and milk chocolate samples.
Qualitative and quantitative Raman spectroscopy was successfully performed directly on chocolate samples.
The technique demonstrated feasibility for analyzing chocolate composition and structure.

Conclusions:

1000 nm Raman spectroscopy is a viable technique for analyzing chocolate, overcoming previous fluorescence challenges.
This method offers a powerful tool for both laboratory research and in-process monitoring of chocolate quality.
The findings support the application of shortwave infrared Raman spectroscopy in the food industry.