Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

324
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
324
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Master Transcription Regulators02:23

Master Transcription Regulators

7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Positive Regulator Molecules02:39

Positive Regulator Molecules

5.6K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid Flippase Mediated Membrane Asymmetry Governs Extracellular Vesicles Biogenesis and Host Interactions in <i>Cryptococcus neoformans</i>.

bioRxiv : the preprint server for biology·2026
Same author

Ovoid cell is an inducible small-sized morphotype that enhances proliferation and antifungal drug tolerance in the human fungal pathogen Cryptococcus neoformans.

PLoS pathogens·2026
Same author

Mannoprotein Cig1 contributes to the immunogenicity of a heat-killed F-box protein Fbp1 <i>Cryptococcus neoformans</i> vaccine model.

Infection and immunity·2025
Same author

Genetic and environmental control of parasexual reproduction in the pathogenic <i>Candida</i> species.

Microbiology and molecular biology reviews : MMBR·2025
Same author

The emerging fungal pathogen Cryptococcus gattii: Epidemiology, pathogenesis, immunomodulatory attributes, and drug susceptibility.

PLoS neglected tropical diseases·2025
Same author

Cryptococcosis, tuberculosis, and a kidney cancer fail to fit the atherosclerosis paradigm for foam cell lipid content.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same journal

Correction: Thaochan et al. Fungal-Infected Weeds: A Potential Source of Leaf Spot Disease in Rubber Trees from Southern Thailand. <i>J. Fungi</i> 2025, <i>11</i>, 220.

Journal of fungi (Basel, Switzerland)·2026
Same journal

Distinct Phenotype and Secondary Metabolite Profile Mark a Dominant <i>Aspergillus flavus</i> Outbreak Strain.

Journal of fungi (Basel, Switzerland)·2026
Same journal

Morphology, Chemistry, and Phylogeny Reveal Two New Species of the Genus <i>Lecidella</i> (Ascomycota, Lecanoraceae) from Anhui Province, China.

Journal of fungi (Basel, Switzerland)·2026
Same journal

Unlocking the Biocontrol Potential of Indigenous Soil Fungi: High-Performing Strains of <i>Beauveria bassiana</i> and <i>Metarhizium robertsii</i> Against the Tomato Leafminer <i>Tuta absoluta</i>.

Journal of fungi (Basel, Switzerland)·2026
Same journal

Unraveling CARD9 Mutations in Deep Dermatophytosis: A Genetic Gateway to Fungal Invasion and Immune Dysfunction.

Journal of fungi (Basel, Switzerland)·2026
Same journal

Draft Genome and Comparative Analysis of a <i>Cutaneotrichosporon jirovecii</i>-Related Yeast Recovered from a Human Fecal Sample.

Journal of fungi (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Aug 16, 2025

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
07:32

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

10.1K

Multiple F-Box Proteins Collectively Regulate Cell Development and Pathogenesis in the Human Pathogen Cryptococcus

Chengjun Cao1, Yina Wang1, Samantha L Avina1

  • 1Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA.

Journal of Fungi (Basel, Switzerland)
|December 22, 2022
PubMed
Summary
This summary is machine-generated.

Researchers studied F-box proteins in the pathogenic fungus Cryptococcus neoformans. These proteins are crucial for the ubiquitin-proteasome system (UPS), impacting fungal growth, stress response, and virulence.

Keywords:
Cryptococcus neoformansE3 ligaseF-box proteinfungal pathogenesisstress response

More Related Videos

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

15.0K
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
08:08

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

7.4K

Related Experiment Videos

Last Updated: Aug 16, 2025

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
07:32

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

Published on: March 19, 2015

10.1K
Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

15.0K
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
08:08

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Published on: January 11, 2018

7.4K

Area of Science:

  • * Molecular Biology
  • * Mycology
  • * Cell Biology

Background:

  • * The ubiquitin-proteasome system (UPS) regulates intracellular protein degradation, impacting diverse cellular processes.
  • * UPS is vital for pathogenic fungal development and virulence.
  • * F-box proteins are key components of SCF (Skp1-Cullin-F-box protein) ligases, recognizing specific protein substrates for degradation.

Purpose of the Study:

  • * To identify and characterize the F-box protein family in *C. neoformans*.
  • * To investigate the roles of F-box proteins in fungal development, stress response, and virulence.
  • * To elucidate the function of specific F-box proteins (Fbp4, Fbp8, Fbp11) in detail.

Main Methods:

  • * Identification of 20 F-box proteins in *C. neoformans*.
  • * Generation of deletion mutants for 19 F-box proteins.
  • * Comprehensive phenotypic analysis of F-box protein mutants.

Main Results:

  • * F-box proteins in *C. neoformans* regulate diverse functions including stress response, cell size, sexual reproduction, antifungal drug resistance, and virulence.
  • * Detailed characterization revealed significant roles for Fbp4, Fbp8, and Fbp11.
  • * The study provides a broad overview of the F-box gene family in *C. neoformans*.

Conclusions:

  • * F-box proteins are essential regulators of *C. neoformans* cellular functions and pathogenesis.
  • * Understanding the fungal SCF E3 ligase-mediated UPS is critical for deciphering fungal development and virulence.
  • * This research lays the groundwork for future studies on UPS-mediated regulation in pathogenic fungi.