Acinetobacter tibetensis sp. nov., Isolated from a Soil Under a Greenhouse in Tibet

A Gram-stain-negative, light yellow, aerobic, non-motile, short rod-shaped bacterium named strain Y-23^T with iprodione-degrading capability was isolated from a soil under a greenhouse in Tibet, PR China. Strain Y-23^T grew at 4-37 ℃ and pH 5.0-9.0 (optimum, 25 ℃ and pH 7.0) with 0-3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and chromosome genome indicated that strain Y-23^T formed a stable evolutionary branch with Acinetobacter tandoii DSM 14970^T. The 16S rRNA gene similarity, digital DNA-DNA hybridization and average nucleotide identity values between strain Y-23^T and Acinetobacter tandoii DSM 14970^T were 98.31%, 43.2% and 91.2%, respectively. The genome size was 3.39 Mbp with a genomic DNA G+C content of 40.59 mol%. The predominant fatty acids were C_18:1 ω9c, Summed feature 3 (C_16:1 ω7c/C_16:1 ω6c), C_12:0, C_12:0 3-OH and C_16:0. The polar lipids were diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline, unidentified phospholipid, four unidentified aminophospholipids and two unidentified lipids. The isoprenoid quinone was Q-8 (19.43%) and Q-9 (80.57%). Based on phenotypic, phylogenetic, and genotypic data, strain Y-23^T is considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter tibetensis sp. nov. is proposed. The type strain is Y-23^T (= CICC 25150^T = JCM 35630^T).