Related Experiment Video
Updated: Aug 16, 2025

08:30
Characterization of Aquatic Biofilms with Flow Cytometry
Published on: June 6, 2018
9.1K
Biofilm and Rivers: The Natural Association to Reduce Metals in Waters
Nicoletta Guerrieri1, Laura Fantozzi1, Andrea Lami1
1National Research Council, Water Research Institute, Largo Tonolli 50, I-28922 Verbania Pallanza, Italy.
Toxics
|December 22, 2022
Summary
Riverbed biofilms act as natural filters, accumulating metals and macroions. Their community composition and pigment content vary seasonally, indicating their potential as bioindicators for river water quality monitoring.
Area of Science:
- Environmental Science
- Ecology
- Biogeochemistry
Background:
- Riverbed biofilms are crucial microhabitats influencing ecosystem services.
- Biofilm composition is dynamic, responding to environmental conditions and water quality.
- Biofilms contribute to biogeochemical cycles and pollutant reduction.
Purpose of the Study:
- To assess the metal and macroion accumulation capacity of riverbed biofilms.
- To investigate the seasonal variability of biofilm community composition and pigment content.
- To evaluate the potential of biofilms as bioindicators for river water quality.
Main Methods:
- Morphological analysis for diatom and non-diatom algal communities.
- Automated mercury analysis and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for metal and macroion quantification.
- High-Performance Liquid Chromatography (HPLC) for carotenoid and chlorophyll analysis.
Main Results:
- Significant seasonal patterns observed in biofilm metal and macroion levels.
- Distinct seasonal differences in biofilm community structure and carotenoid composition.
- Biofilm metal accumulation varied across different sample points and seasons.
Conclusions:
- Riverbed biofilms exhibit seasonal variations in community and accumulated pollutants.
- Biofilm characteristics suggest their utility as bioindicators for monitoring river water quality.
- Further research can refine biofilm-based water quality assessment methods.
Keywords:
arsenicbiofilmextruded polymeric substanceinductively coupled plasma mass spectrometrymercurymetalsoxidative stresswater qualityMore Related Videos
Related Concept Videos
Biofilms
185
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
185
Bioremediation
19.7K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
19.7K
Coagulation
351
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
351
Environmental Applications of Microorganisms
129
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
129
Extraction: Advanced Methods
506
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
506
Microbial Nutrition
174
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
174

